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Unformatted text preview: ix. Once spots are dry, roll the paper into a cylinder and staple ends 1. 2cm staple from top 2. 1.5cm staple from bottom x. Handle paper by edges, place paper in the development chamber. xi. The spots should be just above the layer developing solvent 1. Don’t let the paper touch the walls. xii. Tightly seal and let stand xiii. After the developing solution has moved 3/4 th the way up the strip xiv. Remove paper cylinder xv. Remove staples and lay flat on paper towel to dry xvi. MARK THE POSITION OF SOLVENT FRONT WITH A PENCIL xvii. Once paper dries, outline the colored spots with a pencil and make a dot in the center of each. 1. Center of the Head, not the Tail xviii. Measure the distance in (mm) from starting line of the dot until the center xix. Measure the distance of the starting line to the solvent front. xx. Tape chromatogram to report sheet. xxi. Calculate Rf value for each component....
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