22_Prokar_Transcription (2)

22_Prokar_Transcription (2) - Making RNA from DNA:...

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Unformatted text preview: Making RNA from DNA: Transcription coding strand It is important to know some nomenclature before we get started 1 4 3 2 5 5 and 3 refer to the carbon positions on the sugar ring of RNA or DNA This hydroxyl group is required for RNA polymerization (coding strand) RNA is always synthesized 5 to 3 Therefore, the template strand is read 3 to 5 Transcription occurs in a transcription bubble coding strand coding strand So, in summary: There is an error in the figure below Can you find it ? (From the previous edition of your text) cAMP CAP Regulation of Prokaryotic Transcription Catabolite Activating Protein bound to DNA There was an error in the previous edition of your textbook! Can you find it in Panel A below ? 5 3 5 3 They've got RNA right (5' to 3'), but they have the template being read 5' to 3' (not 3' to 5'). Review In biology, information flows from DNA RNA Protein Replication DNA Polymerase Transcription Translation RNA Polymerase Ribosome Process in Black, Enzyme in Blue Enzymes are proteins that catalyze a specific biochemical reaction Review coding strand It is important to know some nomenclature before we get started Pribnow box E. coli promoters contain characteristic DNA sequences that associate with sigma factors and RNA Polymerase holoenzyme Different sigma factors allow RNA Polymerase to recognize different sets of promoters (p. 378-9) Figure 8-7 Mutations in -10 and -35 sequences affect transcription In both prokaryotes and eukaryotes, transcription proceeds in...
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22_Prokar_Transcription (2) - Making RNA from DNA:...

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