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Recitation week 8 (1)

Recitation week 8 (1) - Ligase 1 Resolving to get CO 5’...

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Recitation 03 / 02 / 2011
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Recombination Initiation After the replication fork has passed, Spo11 makes a double stranded break in the DNA of one of the homologous chromosomes Spo11 stays bound to the 5’ ends to protect and recruit factors needed for recombination
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Recombination - exonuclease Trims  ~1 kb
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Recombination – Strand Invasion ssDNA bound by  Rad51 (RecA)&  Dmc1.  Searches only  homologs, not sisters
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DNA Synthesis D-loop
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Ligation of nicks
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Branch Migration
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The Holliday Junction - RuvABC Helicase Resolvase
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Early Crossover Decision Model to explain # of chiasmata less than # of double strand  breaks
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1. Step 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’
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2. Step 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ Spo11
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3. Step 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ Exo- nuclease
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4. Step 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’
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5. Step 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’
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6. Step 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ DNA-Pol
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6. Step 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’
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Unformatted text preview: Ligase 1. Resolving to get CO 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ vertical horizontal 2. Resolving to get CO 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ vertical horizontal 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ 1. Resolving to get NCO 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ vertical vertical 2. Resolving to get NCO 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ horizontal 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ Summary • CO – Vertical / horizontal – Horizontal / vertical • NCO – Vertical / vertical – Horizontal / horizontal 5’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ vertical vertical horizontal Enjoy SPRING BREAK!...
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