Recitation week 11

Recitation week 11 - Recitation 03 30 2011 Imprinting...

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Recitation 03 / 30 / 2011
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Imprinting results from pre-programmed differences in DNA methylation of selected  genes in the sperm and egg Dad’s wt allele is inactive, Mom’s is active
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Imprinting and  determination make  organismal cloning  difficult Cloning success drops dramatically as the age of the nuclear donor increases
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Dolly http://www.theintellectualdevotional.com/blog/2011/01/page/3/
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Position Effect Variegation: when your  neighborhood matters
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Topics:  Imprinting  Dosage Compensation  Position Effect Variegation  Chromatin Remodeling  A Histone Code? Throughout biology, chromatin is used to regulate  transcription and access to the DNA template
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Histone Modifications A Histone Code? Chromo Domain Bromo Domain   Affect Transcriptional Activity   Recruit Proteins to Specific Loci   Demarcate Functionally Distinct Genomic Regions Khan and Hampsey, Trends Genet. 2002 Aug;18(8):387-9.  Luger et al.  Nature . 1997 389(6648):251-60. Richmond TJ, Davey CA.  Nature . 2003  423(6936):145-50.
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Factors that move nucleosomes  interact with factors that modify  nucleosomes to regulate  template accessibility Pol II recruited, but cannot initiate TBP bound, now Pol II can go The beta-interferon promoter
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RNA Processing, including capping  and splicing , is co- transcriptional
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Capping 7-methyl-G in a 5’ to 5’ triphosphate linkage Capping protects the RNA from degradation, and is  required for translation
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Polyadenylation polyadenylation signal Polyadenylation signals that the transcript is complete,  and protects it from degradation (~250)
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This note was uploaded on 06/27/2011 for the course BIOL 202 taught by Professor Kieber-hogan during the Spring '08 term at UNC.

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Recitation week 11 - Recitation 03 30 2011 Imprinting...

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