Ch.13 HW ans key - Ch 13 HW ans key MCQ 1 2 3 4 5 6 7 B A C A B D B True/False 1 2 3 4 5 F T F T F Match A with B 1 2 3 4 5 6 7 SQ 1 Southern blot

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Ch. 13 HW ans key MCQ: 1) B 2) A 3) C 4) A 5) B 6) D 7) B True/False: 1) F 2) T 3) F 4) T 5) F Match A with B: 1) g 2) f 3) e 4) a 5) c 6) d 7) b SQ: 1) Southern blot: DNA to be probed is cut with restriction enzyme and then the fragments are separated by gel electrophoresis. Alkali treatment of the gel denatures the DNA, which is then blotted onto the nylon or nitrocellulose membrane. A labeled probe (RNA or DNA) is then hybridized to complementary fragments on the filter. Northern blot: RNA is separated in the gel and probed with the labeled DNA 2) A vector is a vehicle to carry recombinant DNA molecules into the host cells where independent replication can occur. Three common vectors are; plasmid, bacteriophages and cosmid.
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An ideal vector should have; 1) The capability to replicate inside the host cell 2) Should contain several restriction enzyme sites 3) Should be able to carry a large piece of DNA 4) Should carry a marker gene to identify the cells that have taken up the vector,
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This note was uploaded on 07/04/2011 for the course BIO 325 taught by Professor Saxena during the Fall '08 term at University of Texas at Austin.

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Ch.13 HW ans key - Ch 13 HW ans key MCQ 1 2 3 4 5 6 7 B A C A B D B True/False 1 2 3 4 5 F T F T F Match A with B 1 2 3 4 5 6 7 SQ 1 Southern blot

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