Chapter 20 - ii. Restriction fragments 1. Sticky ends iii....

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Chapter 20 DNA Technology I. DNA Technology = “Biotechnology” a. Tools that have evolved to aid in the study of DNA II. Gene Cloning a. Figure 20.3 i. Plasmid DNA is isolated 1. Plasmid = extrachromosomal circular DNA ii. gene of interest is inserted 1. resulting plasmid = recombinant molecule iii. recombinant plasmid is returned to bacteria iv. transformed bacterial cell is grown to replicate the foreign molecule v. colonies that contain the recombinant are identified b. TOOLS for gene cloning i. Restriction Enzymes
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Unformatted text preview: ii. Restriction fragments 1. Sticky ends iii. DNA Ligase III. Using nucleic acid probes to identify a cloned gene a. Figure 20.4 IV. complementary DNA (cDNA) a. Figure 20.5 V. Polymerase Chain Reaction (PCR) a. Figure 20.7 b. 1985 – VI. Gel electrophoresis a. Figure 20.8 VII. Southern Blot a. Figure 20.10 b. DNA probed with radiolabeled ssDNA c. Northern Blot, DNA probed with RNA d. Western Blot, protein probed with protein VIII. DNA Sequencing a. Dideoxy-chain termination (Sanger method)...
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This note was uploaded on 07/08/2011 for the course BIOL 101 taught by Professor Smith during the Spring '11 term at Aachen University of Applied Sciences.

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Chapter 20 - ii. Restriction fragments 1. Sticky ends iii....

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