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Unformatted text preview: ii. Restriction fragments 1. Sticky ends iii. DNA Ligase III. Using nucleic acid probes to identify a cloned gene a. Figure 20.4 IV. complementary DNA (cDNA) a. Figure 20.5 V. Polymerase Chain Reaction (PCR) a. Figure 20.7 b. 1985 – VI. Gel electrophoresis a. Figure 20.8 VII. Southern Blot a. Figure 20.10 b. DNA probed with radiolabeled ssDNA c. Northern Blot, DNA probed with RNA d. Western Blot, protein probed with protein VIII. DNA Sequencing a. Dideoxy-chain termination (Sanger method)...
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