Origin of US West Nile virus

Origin of US West Nile virus - REPORTS 4. 5% CO2. Cells...

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5% CO 2 . Cells were examined for cytopathologic effect for up to 7 days after inoculation. 4. C. G. Sibley and B. L. Monroe Jr., Distribution and Taxonomy of Birds of the World ( Yale Univ. Press, New Haven, CT, 1990). Common and scientific names of birds are used in accordance with those listed in this book. 5. Total numbers of mosquitoes by species that were collected in 14 towns in Fairfield County, CT, and tested for virus from 6 September through 14 Octo- ber 1999: Ae. vexans , 1688; Ae . cinereus , 172; Ae . trivittatus , 131; Ae . taeniorhynchus , 123; Ae . sollici- tans , 109; Ae. cantator , 63; Ae . triseriatus , 28; Ae. japonicus , 19; Ae . canadensis ,1 ; Anopheles puncti- pennis , 82; An . quadrimaculatus ,4 ; An . walkeri ,2 ; Coquillettidia perturbans , 15; Culex pipiens , 744; Cx . restuans , 27; Cx . erraticus ,4 ; Cx . territans ,1 ; Culiseta melanura , 76; Cs . morsitans ,1 ; Psorophora ferox ,4 ; and Uranotaenia sapphirina , 104. 6. M. Z. Ansari, R. E. Shope, S. Malik, J. Clin. Lab. Anal . 7 , 230 (1993). Isolates were tested initially in an ELISA against reference antibodies to six viruses, in three families, isolated from mosquitoes in North America. The antibodies were prepared in mice and provided by the World Health Organization Center for Arbo- virus Research and Reference, Yale Arbovirus Re- search Unit, Department of Epidemiology and Public Health, Yale University School of Medicine. The an- tibodies were to Eastern Equine Encephalomyelitis, Highlands J, Cache Valley, LaCrosse, Jamestown Can- yon, and St. Louis Encephalitis viruses. 7. Most dead birds were collected by state or town per- sonnel in Connecticut and sent to the Pathobiology Department at the University of Connecticut, Storrs, where they were examined for postmortem and nutri- tional condition, gross lesions, and microscopic evidence indicative of encephalitis. Brain tissue from birds with presumed encephalitis were frozen at 2 70 o C and then sent to the Connecticut Agricultural Experiment Sta- tion, New Haven, for virus testing. Corresponding brain sections were processed for histologic examination. A 10% suspension of each sampled brain tissue was pre- pared in 1.5 ml of phosphate-buffered saline by tritu- rating with a mortar and pestle ( 3 ). Two to seven tissue samples from each brain were tested for virus. Alundum was added to facilitate homogenization of tissue. Sus- pensions were centrifuged at 520 g for 10 min. The supernatant of each sample was then passed through a 0.22- m m filter before inoculation of a 100- m l sample onto a monolayer of Vero cells. Cells were grown and
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This note was uploaded on 07/12/2011 for the course BIO 620 taught by Professor Hardy during the Spring '11 term at University of Florida.

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Origin of US West Nile virus - REPORTS 4. 5% CO2. Cells...

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