Chpt12_ANS3319_IVF_Lab_10

Chpt12_ANS3319_IVF_Lab_10 - ANS 3319C Reproductive...

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This handout was developed by former Animal Sciences graduate students Jeremy Block and Luiz Augusto (Guto) de Castro e Paula. Jeremy and Guto received their PhD in reproductive physiology in the Department of Animal Science at the University of Florida. This handout was prepared using material from Dr. Pete Hansen’s web page on production of bovine embryos http://www.animal.ufl.edu/hansen/IVF/default.htm . ANS 3319C Reproductive Physiology and Endocrinology Techniques for In-Vitro Embryo Production Objectives 1) To gain an understanding of the process of in-vitro embryo production in cattle and other domestic species. 2) To provide hands-on experience in the techniques involved in in-vitro embryo production including oocyte aspiration and collection, maturation, fertilization, and embryo culture. Application of In-Vitro Embryo Technologies to Domestic Animal Species 1) Production of embryos from genetically valuable animals that are either infertile or that are deceased. 2) An alternative means to produce embryos from valuable animals rather than using superovulation 3) An inexpensive method for producing embryos using abattoir-derived ovaries Overview Although the production of domestic animal embryos in vitro is still a relatively new technique in commercial and clinical settings, work on in vitro fertilization began as early as the 1930’s with rabbit oocytes. While these first attempts at in vitro fertilization were not successful, subsequent research in the late 1950’s led to the birth of rabbit pups produced using oocytes fertilized in vitro. In the late 1960’s, work with human oocytes led to the birth of the first baby, Louise Brown (Figure 1), following in vitro fertilization in the United Kingdom in 1978. Since this time the production of human preimplantation embryos in vitro has become a common treatment for infertility. It is estimated that over 100,000 babies have been born using this technique in the United States alone. In terms of in vitro embryo techniques in domestic animals, the first calf (Figure 1), lambs and pigs produced following in vitro fertilization were born in the early 1980’s and the live birth of a foal following in vitro fertilization was reported in the early 1990’s. The production of bovine embryos in vitro has been the most successful of all the domestic animal species. Research on this topic in the 1980’s led to improved in vitro maturation medium and also techniques for capacitation of sperm in vitro. In the 1990’s new developments led to improved embryo culture conditions such that perimplantation bovine embryos could be cultured to the blastocyst stage in vitro. Over the last 10-15 years, the production of bovine embryos in vitro for commercial use has increased significantly and now there are several hundred thousand bovine in vitro produced embryos transferred worldwide each year. The production of embryos in vitro of other domestic species, especially the pig and the horse, have been less successful and
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Chpt12_ANS3319_IVF_Lab_10 - ANS 3319C Reproductive...

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