MT2 W11 section002

MT2 W11 section002 - NAME_ MIDTERM 2 – WINTER 2011...

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Unformatted text preview: NAME:__________________________________________________________________________________________ MIDTERM 2 – WINTER 2011 SECTION 002 READ EACH QUESTION CAREFULLY. PICK THE BEST POSSIBLE ANSWER Gas Constant: 8.314 J/mol.K or 1.98 x 10 ­3 kcal/mol.K 1) In uncompetitive inhibition: (5 pts) a) I combines only with ES b) I combines only with E c) I combines with E and ES d) I combines with EP e) None of the above 2) Which of the following indicates a system at equilibrium? (5 pts) a) ΔG > 0 b) ΔG = ΔH + TΔS c) ΔG = 0 d) ΔG = RTln([products]/[reactants]) e) none of the above 3) Hydrogen bonding between the protein backbone carbonyl oxygen and hydrogen of the amide nitrogen is distorted (bent) in which of the following? (5 pts) a) protofibrils b) anti-parallel B sheets c) alpha helix d) filaments e) parallel B sheets 4) What is the K’eq for a reaction when ΔGo’ is + 5 kcal/mol? (10 pts) a) b) c) d) e) 2 3.68 x 10 3 4.79 x 10 -1 18.62 x 10 -4 2.09 x 10 -5 5.02 x 10 5) At very high [S], when [S] is considerably greater than Km, v = Vmax. The rate of this reaction is said to be: (10 pts) a) zero order b) first order c) second order d) proportional to [E]t e) dependent on Km/Vmax 6) The enzyme Lacrymatory factor synthase uses which of the following as its substrate: (5 pts) a) allinase b) Prensco propenyl sulphenic acid c) Thiosulphinate d) Propanethial S-oxide e) None of the above 7) Proline, and which of the following make up a large percentage (approximately 30%) of the amino acid composition in tropocollagen: (5 pts) a) hydroxy proline b) lysine c) phenylalanine d) alanine e) none of the above 8) The central rod domain of a keratin protein is approximately 95 residues in length. The length of this keratin domain in nanometers is: (10 pts) a) 46.8 b) 134.6 c) 13.5 d) 47.0 e) 95.0 9) For the following Lineweaver Burk plot, what is the correct kinetic term for the point labeled “A” (10 pts). a) 1/Vmax b) -1/Km c) 1 + ([I])/Ki’) d) 1 + ([I]/Ki)/Vmax e) (1 + ([I]/Ki’)/Vmax Consider the Michaelis Menten kinetic data presented below and answer each of the following questions ( 10  ­ 14) Vmax (mmol/ml.min) (or Vmax app) Km (or Kmapp) (mM) No Inhibitor 35.0 5.0 [I]1 = 10 mM 20.0 12.0 [I]2 = 10 mM 35.6 10.0 [E]t = 10 ­6 M 10) What type of inhibition is exhibited by Inhibitor #1? (10 pts) a) competitive b) non-competitive c) mixed d) uncompetitive e) double displacement 11) What type of inhibition is exhibited by Inhibitor #2? (10 pts) a) competitive b) non-competitive c) mixed d) uncompetitive e) ping-pong 12) What is the Ki for Inhibitor #2? (15 pts) a) 5 mM b) 10 mM Kmap = Km (1 + [I]/Ki) c) 10 µM d) 20 mM e) 1.0 mM 13) In the absence of inhibitor, under conditions of initial velocity, and at saturating substrate concentrations, what is the turnover number for this system? (10 pts) a) 3.5 x 107/min b) 3.5 x 107/sec c) 5.8 x 105/min k2 = Vmax/[E]t = kcat d) 1.0 x 10-6/sec e) 7.0 x 106/min 14) What is k2 for this system as described in problem #13 above? (10 pts) a) 1 x 107/min b) 3.5 x 107/sec same answer, but convert to seconds c) 5.8 x 105/sec d) 1.0 x 10-6/sec e) 7.0 x 106/min 15) The standard state free energy of hydrolysis of ATP is  ­30.5 kJ/mol ATP + H2O  ­ ­ ADP + Pi In mammalian blood cells, the concentrations of ATP, ADP and Pi are 2.25, 0.25, and 1.65 mM, respectively. For simplicity, consider this reaction to take place at a pH of 7.0 and 25oC. What is the free energy change in kJ/mol for this reaction? (15 pts) a) -4.21 x 10-3 b) + 3.68 x 10-3 ΔG’ = ΔGo’ + RTln[Products]/[reactants] c) d) -52 e) +42 f) none of the above 16) What type of reaction mechanism is shown in the graph below? (10 pts) a) b) c) d) e) pure non-competitive inhibition double displacement bisubstrate mixed non-competitive inhibition random single-displacement bisubstrate ordered single-displacement bisubstrate 17) Fatty acids are often described by a “symbol” denoting the number of carbons and double bonds. Which of the following common biological fatty acids is denoted by the symbol “18:2”? (5 pts) a) Linolenic acid b) Myristic acid c) Palmitic acid d) Stearic acid e) Linoleic acid ...
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This note was uploaded on 07/13/2011 for the course BIS 102 taught by Professor Hilt during the Winter '08 term at UC Davis.

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