exp8r - Experiment # 8 Molecular Biology 04/20/10 Biol...

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Experiment # 8 Molecular Biology 04/20/10 Biol 111-01 Introduction
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Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field. It is ultimately caused by the presence of a charged interface between the particle surface and the surrounding fluid. Genomics is the study of DNA, the genomes is the entire library of genetic material of an organism. Recombinant DNA, is taking a gene from one and putting it in another on. Purpose The lab is done to teach students about Molecular Biology, to show students how to properly use the materials in the lab and achieve correct results. Materials Gel electrophoresis ice Gel plates restriction enzymes: Comb to make walls A v a II, P v u II Chamber for electrophoresis restriction buffers Chamber cover molecular weight markers Power supply with electrodes (DNA) 250 mL Erlenmeyer flask gel loading dye (bromophenol blue) 100 mL graduated cylinder plastic wrap Microwave or hot plate 15 1.5 mL microtubes (various colors) Deionized water thermometer Agarose metric rulers pUC 19 (plasmid) DNA TBE (Tris base, boric acid, ethylenediaminetetraacetic acid Polaroid camera with polaroid 667 film microcentrifuge micropipettors and tipr 55 C water for agarose 37 C incubator or water bath Portable freezer Methylene blue stain: alternative stain:
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0.025% methylene blue
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exp8r - Experiment # 8 Molecular Biology 04/20/10 Biol...

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