Experiment3 - Experiment#3 Enzymes 03/29/10 Biol11102...

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Experiment #3 Enzymes 03/29/10 Biol 111-02 (revised)
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Introduction Enzymes are biological catalysts, compounds that speed up a chemical reaction without being used up or altered in the reaction. Enzymes bind with substrates at their active site, forming the enzyme-substrate complex. Enzymes are mainly proteins; they catalyze all aspects of cell metabolism, including the digestion of food, in which large nutrient molecules (including proteins, carbohydrates, and fats) are broken down into smaller molecules, the conservation and transformation of chemical energy, and the construction of cellular materials and components. Purpose This experiment will teach us students the difference between activators and cofactors, know that the function of an enzyme is based on its shape and configuration. We will also be able to recognize the difference between competitive and noncompetitive inhibition and where the different inhibitors will bind. Materials 1 reaction spot plate Prepared starch solution 2 small cups Prepared diastase solution 3 plastic pipettes Distilled water Clock w/ second hand 1 bottle of starch indicator solution Bottle of dilute hydrochloric (HCI) solution 0.1 % Bottle of dilute sodium hydroxide (NaOH) solution 0.1% Test tubes of enzyme solution at varying temperatures
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This note was uploaded on 07/13/2011 for the course BIOL 131 taught by Professor Rahmadan during the Spring '08 term at Texas Southern.

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Experiment3 - Experiment#3 Enzymes 03/29/10 Biol11102...

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