KrebsCycleI2010SRS

KrebsCycleI2010SRS - Citric Acid Cycle 1 Lecture 26 Chapter...

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Citric Acid Cycle 1 Lecture 26 Chapter 17-3
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Figure 17-2
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Citric Acid Cycle Other names: Krebs cycle, Tricarboxylic Acid Cycle (TCA). Circular pathway: all intermediates are “catalytic” (no net formation or consumption). • Complete oxidation of Acetate to CO 2 . 8 steps, 4 of which are oxidations. Electrons will be transferred into electron transport to generate ATP through Ox. Phos.
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Balanced equation: Acetyl-CoA + [3 NAD + + FAD] + GDP + Pi --> (substrate) (electron acceptors) (substrate level P-lation) 2 CO 2 + [3 NADH + FADH 2 ] + GTP + CoA (product) (electron donors)
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Figure 17-2
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Reaction 1: Citrate Synthase Acetyl-CoA + OAA + H 2 O ---> Citrate + CoA Coupled reaction: Condensation of methyl group of Acetate to carbonyl of OAA (unfavorable). Hydrolysis of thioester (favorable). Overall reaction favorable. Irreversible in cells. Bisubstrate reaction with ordered binding (OAA first) prevents premature hydrolysis of thioester.
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Figure 17-9 Open form, binds OAA: Closed form, binds Acetyl-CoA: Ordered binding ensures Acetyl-CoA only binds with OAA is present. Hexokinase works the same way.
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Page 578 Reaction 2: Aconitase Reversible isomerization of citrate and isocitrate. Enzyme bound intermediate: cis-Aconitate Contains an Iron-Sulfur cluster for binding substrate.
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Figure 17-2 Aconitase moves -OH away from the carbons which were from Acetyl CoA. Citrate is a symmetric molecule. How does aconitase do this?
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Page 325
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Citrate is a prochiral molecule (replacement of either -CH 2 COO - groups would make central C chiral). Enzyme is asymmetric,
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KrebsCycleI2010SRS - Citric Acid Cycle 1 Lecture 26 Chapter...

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