Lec 6 Bacterial Genetics

Lec 6 Bacterial Genetics - Genetics of Bacteria and...

Info iconThis preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon
Genetics of Bacteria and Bacteriophage Basic features and methods for genetic analysis Prokaryotic sex: types of DNA exchange Transformation • Conjugation • Transduction • Phage co-infection Genetic mapping Lysogeny: site-specifi integration of bacteriophage lambda into the E. coli chromosome
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Bacterial Growth cell wall circular chromosome plasma membrane septum + Total time: 20 minutes E. coli nutritional requirements 1. Carbon source 2. Phosphate, sulfate 3. “Fixed” Nitrogen (e.g., NH 4 ) 4. Trace metals (Mg ++ , Cu ++ , etc.) = minimal media
Background image of page 2
Laboratory Growth of E. coli 1. Flasks 2. Test tubes 3. Petri dishes cotton plug solid agar media >10 9 /ml colony ~ 10 6 cells dilution overnight Count number of colonies to determine titer of viable cells in inoculating culture
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Bacteriophage infection cycle
Background image of page 4
Plaque assay measures phage titer (phage/ml) 10-fold serial dilutions phage suspension add E. coli, spread on plates “lawn” of E. coli confluent lysis plaque initiated by single phage
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Typical Bacterial Phenotypes Defect in a nutritional requirement WT his - Minimal media Minimal + histidine growth no growth growth (prototroph) growth (auxotroph) Ability to utilize specific sugars WT gal - sugar= galactose sugar= glucose growth no growth growth growth Drug resistance WT + -- tet R+ + - kan - + min Min + tetracyline Min + kanamycin Phage resistance WT - ton Growth in presence of Phage T1
Background image of page 6
Figure 5-2 DNA transfer between bacteria
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Mechanism of DNA uptake by bacteria Figure 5-21
Background image of page 8
The biological principle of genetic transformation Mendel – genes are transmitted from parent to progeny (vertical transmission) Griffith – virulence can be transmitted horizontally between pneumococcus bacteria
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 10
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 37

Lec 6 Bacterial Genetics - Genetics of Bacteria and...

This preview shows document pages 1 - 10. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online