exam 2 3 - I RNA Trarrecr'iptlcn and Processing a...

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Unformatted text preview: I. RNA: Trarrecr'iptlcn and Processing a. Errane: 'expremed regisn' that enccde parts cf prcteits. b. lntrcns: 'interveriru regicn' that separate escns. c. RNA. Epllclng: when the splicecscrne remmas the intrcns and jnins Ihe escns tun prcduce mature RNA. that cuntains the cpntinucus itfcrrraticn needed tn synlhesize a protein. d. Alternative Epllclng: prccess Ihat allcws cur Efiflflfl genes tc encdde carer tflflfiflfl prefers a. DNA. and RNA functinn is based en 2 principles: i. [:1:::m|;rlernlentarit'_I..I cf bases determines the sequence cfnew DNA strand in replicaticn. and DNA transcript in trarslaticn- ii- Certait prcteits reccgnize particular base sec-lences in DNA- These nudeic-acid-bintiru prcteins bind tc these and act un thern- ll. RNA a. Pulse-Chase Experiment prctccp-l in 1.rllhicl'l an infected bacteria are fed {pulsed with]: radicactive uracil [used it RNA but nct DNA}- An-_.r RNA syntl‘resized I.-.ril| be 'labeled" 1.illilt'l the radidactivity. After a while it is replaced {chased} with nan-radidactise uracil- This indicates RNA ha a very shcrt lifetime b. Prcpertl-es at DNA: i. RNA is usually a single-stranded nucl-enfide chain {net a dcuble- helix}- 1- This makes it mcre flea-rifle. enabling r'ncre ccrrplex 3d shapes, and album intramnlecular base-palrlng {prairirg with itself)- ii- RNA ha rlbcsl sugar in its nuclectides. rather that decayribdse lire i1 DNA- lt mnsfrcm EDT 1- RNA has CIH cn the 2’4:arbcn rather Ihan a H- ii- RNA nuclectides have the pyrirridine base uracil Instead cf thymlne- 1- The bases U and G furm base pairs unly duritg Rl-«lAfc-lcing, nct during transcriptiun. I. can pdr will truth A and E. iI.r- RNA - litre prctein, can catalyze hlclngical reactlcrre. 1- thnq'n'rl: RNA mclecules that functicn litre prctein enzymes c. Classes cf RNA: i. Messenger RNA [mRHA]:enccdls the lnfnrrnatipn needed tn mire pclypeptide chains [prcteinsl because these RNA's serve like it‘tflm'fldiil‘f {messengers} that passes itfcrrraticn fi'cm DINA tn prctein. ...
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