Lec 10 Translation & Genetic Code

Lec 10 Translation & Genetic Code - Proteins are...

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Figure 3-23 Molecular Biology of the Cell (' Garland Science 2008) Proteins are amazingly diverse in structure
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Figure 3-1 Molecular Biology of the Cell (' Garland Science 2008) All protein structure derives from the primary sequence of amino acids
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The Central Dogma of Molecular Biology nucleus cytoplasm DNA mRNA 5& 5& 3& transcription translation RNA polymerase protein
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A TRANSLATING RIBOSOME
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exit site peptidyl site aminoacyl site E PA 30S subunit 3’ mRNA 5’ anticodon loop aminoacyl tRNA (charged tRNA) 50S subunit peptidyl-tRNA (has growing peptide chain attached) uncharged tRNA direction of translation A TRANSLATING RIBOSOME
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Surface of the ribosome from the bacterium Haloarcula marismortui
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The structure of transfer RNA
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Coupling of amino acids to tRNA OH O C C N CH 3 H H alanine H AMP O C C N CH 3 H H H O O C C N CH 3 H H H ATP P i AMP tRNA Ala Alanyl-tRNA Ala adenylated alanine Alanyl-tRNA synthetase Alanyl-tRNA synthetase 1/40,000 error rate 3’ 5’ 5’ Editing to remove an incorrect amino acid
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-G-G-C-G-C-A- C-C-G O tRNA Gly O C C C N C N H H H H H R O glycine C - G U O O C C N CH 3 H H alanine tRNA Ala H 5& 3& mRNA 5& C O C N R H H Basic features of protein synthesis direction of translation growing peptide chain P site 5& A site
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-G-G-C-G-C-A- C - G O tRNA Gly O C C C N C N H H H H H O glycine C-G-U CH 3 alanine tRNA Ala 5& 3& C O C N R H H new peptide bond
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exit site peptidyl site aminoacyl site E PA 30S subunit 3’ mRNA 5’ anticodon loop aminoacyl tRNA (charged tRNA) 50S subunit peptidyl-tRNA (has growing peptide chain attached) uncharged tRNA direction of translation A TRANSLATING RIBOSOME
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Schmeing & Ramakrishnan (2009) Nature 461, 1234-42
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AUG 5& 3& UAC f-Met GTP IF-3 IF-1 50S subunit AUG 5& 3& UAC f-Met GDP IF-3 IF-2 IF-1 + Pi 70S ribosome Initiation Complex AUG IF-3 30S 5& 3& UAC f-Met IF-1 + IF-2 GTP Translation Initiation in E. coli
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AGGAGG-7nt-AUG UCCUCC Bacteria: Shine-Dalgarno site in mRNA 3 & end 16S rRNA Met A ccAUGg AUG Met Eukaryotes: scanning 1st “good context” AUG FINDING THE INITIATION SITE Shine-Dalgarno mechanism allows for translation
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This note was uploaded on 07/17/2011 for the course BIO 151 taught by Professor Grinblat during the Summer '08 term at University of Wisconsin.

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Lec 10 Translation & Genetic Code - Proteins are...

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