Ex02_Su11-Analysis

Ex02_Su11-Analysis - Group Members_Date LI Unique Day Room...

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LI ______________________ Unique __________ Day: __________ Room: _______ Lab Start Time ________ Exercise 2 Analysis To be completed as a group and turned in at the beginning of your next laboratory period. Include your “Data & Results”, sketches, acquired digital images, etc. as directed by your laboratory instructor. Show your work for all calculations and/or print your MS Excel data sheets. Be sure to include proper units where necessary. Adhere to University’s Honor Code and course policies. Balance between brevity and completeness. (1) Answer the following regarding the Olympus CX31 brightfield microscope you used for this exercise. (a.) Does it have a built-in illuminator or does it have a separate illumination source? The Olympus CX31 brightfield microscope has a built-in illuminator. (b.) Is it a monocular, binocular, or triocular microscope? It is a binocular microscope. (c.) How do adjustments of the field diaphragm affect the quality of a viewed specimen? The field diaphragm controls how much light enters the substage condenser and the rest of the microscope. (d.) How do adjustments of the aperture diaphragm and light intensity affect the view of a specimen? By varying the size of the diaphragm opening, the circle of light projected into the objective is decreased. This decreases the resolution and the image becomes darker. (e.) How does the “field of view” change as magnification is increased? How does this relate to the fact that one should always use the low-power scanning objective first to locate a specimen? The “field of view” decreases as the magnification increases. (f.) What happens to the “depth of focus” as magnification is increased? The “depth of focus” decreases as the magnification increases. (g.) What is used to clean optical surfaces of microscopes and why? Lens paper is used to clean the optical surfaces of the microscopes because it won’t scratch the lenses and the chemicals on the paper won’t deteriorate them either. (2) List the steps required to adjust the microscope for Köhler illumination. To adjust the microscope for Kohler illumination, one must look through the microscope at 100x magnification. Next, raise the condenser all the way and close the field diaphragm so that there is a small circle of light surrounded by a red halo. Then, the height of the condenser must be corrected until a color somewhat between red and blue appears around the edges of the field of light. Finally, the field diaphragm must be opened just enough so that the ring of light becomes its maximum size and the field of view appears round under low power magnification. (3)
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Ex02_Su11-Analysis - Group Members_Date LI Unique Day Room...

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