Lecture 2 Notes - Lab 2 Microscopy: Introduction to the...

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Lab 2 Microscopy: Introduction to the light microscope
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In a scientific experiment, the control group. .. a. is observed more frequently than the experimental group(s). b. is observed less frequently than the experimental groups(s). c. receives all of the experimental treatments being tested. d. receives none of the experimental treatments being tested.
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Objectives of Lab Calculate the resolving power of the lens combinations on the light microscope. How to use your condenser and set Köhler illumination Calibrate the ocular reticle. Review characteristics of bright Feld microscopes. ±amiliarization with elements of computer image analysis. Gather and analyze observational data on paramecium Observe the various stages of mitosis in stained onion root tip cells. Observe living specimens under magniFcation .
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Tools of Observation Initial studies of natural phenomena made with unaided senses, sight, smell, hearing, touch. However, our senses are limited: – We can only detect a narrow section of the electromagnetic spectrum (between 750 nm and 400 nm) Resolution : For unaided human eye, the closest that two objects (or edges) can be together and still distinguished as separate entities ~0.1 mm Tools of observation allow us to extend our senses make us aware of natural phenomena invisible to our senses.
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Optical Microscopes -A microscope is an instrument for viewing objects that are too small to be seen by the unaided eye. -An optical microscope (also called a light microscope) uses light to project a magnified view of the object. Some examples of light microscopes
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I llustration of a Simple Microscope Note: Although light is shown here passing through the specimen, hand lenses are more typically used to focus light is reflected from the surface of an opaque specimen. Specimen (object) Image (magnified view of the object)
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Compound light microscopes that are used to observe cells project light through the specimen. I llustration of a Compound Microscope specimen (object) Image (twice magnified view of the object)
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Brightfeld Microscope 3 Lens System Condenser: gathers light and points it onto specimen Objective lens: Focus light From specimen and creates a magnifed image oF specimen Ocular: magnifes image From objective
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This note was uploaded on 07/18/2011 for the course BIO 206L taught by Professor Unknown during the Summer '08 term at University of Texas at Austin.

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Lecture 2 Notes - Lab 2 Microscopy: Introduction to the...

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