Ex03_Su11-Analysis - Group Members: _Date: _ LI _ Unique _...

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LI ______________________ Unique __________ Day: __________ Room: _______ Lab Start Time ________ Exercise 3 Analysis To be completed as a group and turned in at the beginning of your next laboratory period. Include your “Data & Results”, sketches, acquired digital images, etc. as directed by your laboratory instructor. Show your work for all calculations and/or print your MS Excel data sheets. Be sure to include proper units where necessary. Adhere to University’s Honor Code and course policies. Balance between brevity and completeness. (1) Answer the following questions concerning the use of immersion oil with brightfield micrsocopy. (a.) What is the purpose of immersion oil? The purpose of immersion oil is to increase the resolution so that you can see the cellular features more distinctly. (b.) For the microscopes used in this exercise, which objectives require the use of immersion oil? How can you tell? Only the 100x objective requires the use of immersion oil. One can tell this by looking at the objective and seeing that it says the word “OIL” on it. (c.) If an objective is designated for use with immersion oil, must it be used? Yes, immersion oil must be used if an objective requires it. (d.) Can immersion oil be used with other non-oil-rated objectives? No, immersion oil cannot be used with other non-oil-rated objectives. (e.) Describe the proper way to place immersion oil on a slide and use it with your oil immersion objective. To use immersion oil, first obtain a view of the specimen at a lower magnification and then prepare to switch to the oil immersion lens. As the nosepiece bearing the objectives is turned, place a drop of oil on the slide over the specimen. Then click the oil immersion lens into place. (f.) Why must the focal plane never be adjusted when adding immersion oil? The objective lens, cover slip, and slide could all be crushed. (g.) After immersion oil has been used, what must you do before you can examine a slide again at lower magnification? After immersion oil has been used, you must use lens paper to wipe off the lens. (2) Based on your observations in Section 3.1, compare red and white blood cells. (a.) What cellular features present in white cells? In red blood cells? The white blood cells included nuclei and cytoplasm while the red blood cells did not contain a nucleus. (b.) Which is more prevalent in a typical blood sample –red or white blood cells? How much more prevalent? In a typical blood sample, red blood cells are more prevalent than white blood cells. The ratio in normal blood is 600 red blood cells to each white blood cell. Summer 2011 Ex 3 Analysis
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This note was uploaded on 07/17/2011 for the course BIO 206L taught by Professor Unknown during the Spring '08 term at University of Texas at Austin.

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Ex03_Su11-Analysis - Group Members: _Date: _ LI _ Unique _...

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