test2 - BCH 4053 February 28, 2003 (10) 1. HOUR TEST 2...

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BCH 4053 February 28, 2003 HOUR TEST 2 NAME_____________________ (10) 1. Describe two methods of cleaving disulfide bonds prior to sequencing a protein, indicating the reagents used in each case and the structure of the cysteine residue produced after the cleavage. Page Points 1 ____ 2 ____ 3 ____ 4 ____ Total ______ (6) 2. A peptide was subjected to the following degradative techniques resulting in the fragments with the indicated sequences. I. Cyanogen bromide treatment Asp-Ile-Lys-Gln-Met Lys-Val-Ser Lys-Phe-Ala-Met Tyr-Arg-Gly-Met II. Trypsin hydrolysis Gln-Met-Lys Gly-Met-Asp-Ile-Lys Phe-Ala-Met-Lys Tyr-Arg Val-Ser Give the complete sequence of the original peptide. (6) 3. Fill in the following blanks with a helix or disordered to represent the primary conformation of the indicated synthetic polypeptide at the indicated pH. Polypeptide pH 2 pH 7 pH 12 polyglutamate __________ _________ __________ polylysine __________ _________ __________
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BCH 4053 --Hour Test-2 Page 2 Name________________________
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This note was uploaded on 07/22/2011 for the course BCH 4053 taught by Professor Logan during the Spring '06 term at FSU.

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test2 - BCH 4053 February 28, 2003 (10) 1. HOUR TEST 2...

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