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Neurophysiology exercisess

Neurophysiology exercisess - N europhysiology of Ne rve I...

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Neurophysiology of Nerve Impulses Electrical Stimulation 1. Set the voltage at 1.0 V by clicking the ( _ ) button next to the Voltage display. 2. Click Single Stimulus. Do you see any kind of response on the oscilloscope screen? _yes, flat line__________ If you saw no response, or a flat line indicating no action potential, click the Clear button on the oscilloscope, increase the voltage, and click Single Stimulus again until you see a trace (deflection of the line) that indicates an action potential. What was the threshold voltage, that is, the voltage at which you first saw an action potential? ____3___ V Click Record Data on the data collection box to record your results. 3. If you wish to print your graph, click Tools and then Print Graph. You may do this each time you generate a graph on the oscilloscope screen. 4. Increase the voltage by 0.5 V, and click Single Stimulus. How does this tracing compare to the one trace that was generated at the threshold voltage? (Hint: Look very carefully at the tracings.) depolarization and hyperpolarization were more extreme What reason can you give for the change? More stimuli = higher “firing” Click Record Data on the data collection box to record your results. 5. Continue to increase the voltage by 0.5 V and to click Single Stimulus until you find the point beyond which no
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