midterm - chevrette

midterm - chevrette - BIOLOGY 202 MIDTERM VERSION 1 1 Dr....

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BIOLOGY 202 MIDTERM VERSION 1 1 Dr. Chevrette’s Questions (36 points) 16. (3 points) Which of the following statements is incorrect . a) The length of VNTRs varies between individuals and even in the same individual. b) Because highly repetitive DNA sequences are present at more than 100,000 copies /genome, they will be seen easily on an ethidium bromide stained agarose gel upon digestion of human DNA. c) Cesium chloride gradients can be used to isolate satellite DNA from eukaryotes. d) The C-value paradox defining the lack of correlation between the size of the genome and the biological complexity of an organism can be explained largely by the presence of repetitive DNA. e) Transposable elements, although present in the human genome, are rarely inserted into the exons of the genes. 17. (6 points) The following figure represents an ethidium bromide stained gel containing molecular weight markers (“M”), and fragments of DNA from 5 samples (1 to 5) that have been digested with restriction enzymes, and then separated according to their size. M 3 1 4 2 5 10 kb 8 kb 7 kb 6 kb 4 kb 3 kb 1 kb Based on this gel, which one of the following statements are true . a) This gel could represent the complete digestion of the same plasmid with 5 different restriction enzymes. b) The 1 kb fragment seen in samples 2 and 4 will always be identical and will have the same DNA sequence. c) If one was to use the 10 kb fragment of sample 2 as a probe in a Southern blot obtained from this gel, there will be a hybridization signal only in samples 1 and 2. d) If one was to use the 7 kb fragment of sample 4 as a probe in a Southern blot obtained from this gel, there will always be a hybridization signal in all samples. e) If two of the 5 samples are the same, sample 4 can be obtained by digesting sample 2 with an additional restriction enzyme. 18. (3 points) Which of the following statements is incorrect . a) Slipped mispairing during DNA replication is the main cause of microsatellite polymorphism. b) A rapid analysis of RFLPs among individuals within a population can be performed by combining PCR amplification, restriction enzyme digestion and gel electrophoresis. c)
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This note was uploaded on 07/24/2011 for the course BIOL 202 taught by Professor Y during the Winter '09 term at McGill.

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midterm - chevrette - BIOLOGY 202 MIDTERM VERSION 1 1 Dr....

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