banana - Plant Cell, Tissue and Organ Culture 76: 189191,...

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Plant Cell, Tissue and Organ Culture 76: 189–191, 2004. © 2004 Kluwer Academic Publishers. Printed in the Netherlands. 189 Research note Infuence oF liquid pulse treatment with growth regulators on in vitro propagation oF banana ( Musa spp. AAA) P. Madhulatha, M. Anbalagan, S. Jayachandran & N. Sakthivel Department of Biotechnology, Pondicherry University, Pondicherry 605014, India ( requests for offprints; Fax: +91-413-2655211; E-mail: sakthivelnataraj@hotmail.com) Received 4 July 2002; accepted in revised form 17 June 2003 Key words: banana ( Musa spp. AAA), in vitro propagation, pulse treatment Abstract The effect of liquid pulse treatment of growth regulators on in vitro propagation of banana ( Musa spp. AAA) was studied. Optimal shoot proliferation rates were achieved due to the pulse treatment of 6-benzylaminopurine (BA) and kinetin combination (1:1) at the concentration of 50 mg l 1 for 60 min. Similarly high frequency of root induction was obtained due to pulse treatment with a NAA and IBA combination (1:1) at the concentration of 100 mg l 1 for 60 min. Abbreviations: BA – 6-benzylaminopurine; NAA – α -naphthaleneacetic acid; IBA – indolebutyric acid; MS – Murashige and Skoog Introduction Banana ( Musa spp. AAA), an important fruit crop of the family Musaceae, is widely grown in devel- oping countries. Banana is the second largest fruit crop after citrus (Swennen and Rosales, 1994). India occupies the third place in annual production of ba- nana. Devastating diseases of banana such as black sigatoka, fusarium wilt and banana bunchy top virus caused a total yield reduction of 30–50%. Consider- ing the sterility and polyploidy of the edible banana varieties, Musa breeding remains a difFcult endeav- our (Swennen and Vuylsteke, 1993). Tissue culture propagation of banana has gained attention due to its potential to provide genetically uniform, pest and disease free planting material. In vitro propagation of banana through shoot tip has been demonstrated successfully (Cronauer-Mitra and Krikorian, 1984; Banerjee and de Langhe, 1985; Jarret et al., 1985). Effects of auxins and cytokinins on multiple shoot pro- liferation have been reported (Gleddie et al., 1983). The in±uence of cytokinin pulse treatments on ad- ventitious bud formation on vegetative buds of Piecea abies , Solanum melongena (Magioli et al., 1998) and Pseuditsuga menziesii (Goldfarb et al., 1991) has been studied. However, no previous report has appeared on the use of liquid pulse treatment with combinations of growth regulators for in vitro propagation of banana.
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This note was uploaded on 07/23/2011 for the course HOS 6737c taught by Professor Moore during the Spring '09 term at University of Florida.

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banana - Plant Cell, Tissue and Organ Culture 76: 189191,...

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