Plant Cell, Tissue and Organ Culture
Kluwer Academic Publishers. Printed in the Netherlands.
Infuence oF liquid pulse treatment with growth regulators on
propagation oF banana (
P. Madhulatha, M. Anbalagan, S. Jayachandran & N. Sakthivel
Department of Biotechnology, Pondicherry University, Pondicherry 605014, India (
requests for offprints; Fax:
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Received 4 July 2002; accepted in revised form 17 June 2003
propagation, pulse treatment
The effect of liquid pulse treatment of growth regulators on
propagation of banana (
was studied. Optimal shoot proliferation rates were achieved due to the pulse treatment of 6-benzylaminopurine
(BA) and kinetin combination (1:1) at the concentration of 50 mg l
for 60 min. Similarly high frequency of
root induction was obtained due to pulse treatment with a NAA and IBA combination (1:1) at the concentration of
100 mg l
for 60 min.
BA – 6-benzylaminopurine; NAA –
-naphthaleneacetic acid; IBA – indolebutyric acid; MS –
Murashige and Skoog
spp. AAA), an important fruit crop
of the family Musaceae, is widely grown in devel-
oping countries. Banana is the second largest fruit
crop after citrus (Swennen and Rosales, 1994). India
occupies the third place in annual production of ba-
nana. Devastating diseases of banana such as black
sigatoka, fusarium wilt and banana bunchy top virus
caused a total yield reduction of 30–50%. Consider-
ing the sterility and polyploidy of the edible banana
breeding remains a difFcult endeav-
our (Swennen and Vuylsteke, 1993). Tissue culture
propagation of banana has gained attention due to
its potential to provide genetically uniform, pest and
disease free planting material.
of banana through shoot tip has been demonstrated
successfully (Cronauer-Mitra and Krikorian, 1984;
Banerjee and de Langhe, 1985; Jarret et al., 1985).
Effects of auxins and cytokinins on multiple shoot pro-
liferation have been reported (Gleddie et al., 1983).
The in±uence of cytokinin pulse treatments on ad-
ventitious bud formation on vegetative buds of
(Magioli et al., 1998) and
(Goldfarb et al., 1991) has been
studied. However, no previous report has appeared on
the use of liquid pulse treatment with combinations of
growth regulators for
propagation of banana.