Development 2009 - In Vitro Developmental Pathways Explant...

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In Vitro Developmental Pathways
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Explant - Piece of tissue put into culture -Tissue selection depends on purpose, species, many factors
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Explants Pieces of organs Leaves Stems Roots Cotyledons Embryos Other
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Explants Specific cell types Leaf tissue Embryo Pollen Endosperm Nucellus
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Callus Unorganized, growing mass of cells Dedifferentiation of explant Loosely arranged thinned walled, outgrowths from explant No predictable site of organization or differentiation Auxin + cytokinin Often can be maintained indefinitely by subculture, but may lose ability to redifferentiate Compact vs friable Habituation
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Three stages of callus culture Induction: Cells in explant dedifferentiate and begin to divide Proliferative Stage: Rapid cell division Differentiation stage (sometimes): organogenesis or embryogenesis
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Induction © 1998-2003, Branch of Shemyakin&Ovchinnikov IBCh RAS
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Division E. Sutton, UC Davis
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Callus © 1998-2003, Branch of Shemyakin&Ovchinnikov IBCh RAS
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Differentiation Organogenesis Somatic embryogenesis
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Cell Cultures? Suspension Cultures
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This note was uploaded on 07/23/2011 for the course HOS 6737c taught by Professor Moore during the Spring '09 term at University of Florida.

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Development 2009 - In Vitro Developmental Pathways Explant...

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