GAM 2009 - Applications of Somatic Hybridization and...

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Unformatted text preview: Applications of Somatic Hybridization and Cybridization in Scion and Rootstock Improvement with Focus on Citrus Jude Grosser and Milica alovi University of Florida, Citrus Research and Education Center, Lake Alfred, FL USA- Somatic Hybridization Scion Improvement- building high quality tetraploid breeding parents- triploid recovery from interploid crosses- Somatic Cybridization- transfer of Satsuma CMS (Cytoplasmic Male Sterility) cytoplasm to seedy cultivars for potential seedlessness- Somatic Hybridization Rootstock Improvement- somatic hybridization of complementary parents- building a better sour orange fusion of selected mandarin + pummelo parents SWEET ORANGE EMBRYOGENIC CALLUS SWEET ORANGE ORGANOGENESIS ADVENTITIOUS SHOOT BUD INDUCTION SUCROSE-MANNITOL GRADIENT FOR PROTOPLAST PURIFICATION SWEET ORANGE SUSPENSION CULTURE PROTOPLASTS LEAF-DERIVED CITRUS PROTOPLASTS TYPICAL SUSPENSION PROTOPLAST + LEAF PROTOPLAST PEG-INDUCED FUSION RMAN Ploidy Analysis Showing Diploid and Tetraploid Peaks Using a Partec Tabletop Flow Cytometer NEW SOMATIC HYBRID PLANT NOVA + SUCCARI SOMATIC HYBRID TREE TETRAPLOID SOMATIC HYBRID COMBINATIONS PLANTS REGENERATED FROM EMBRYOGENIC CULTURE + LEAF FUSIONS AT THE CREC: Intergeneric, sexually compatible 41 Intergeneric, sexually incompatible 16 Interspecific, sexually compatible >200 Total: >250 Tacle a seedless triploid mandarin hybrid produced by A. Starrantino. Somatic Hybrid Pollen Parents Used For Mandarin Citrus Fruit Improvement To Date Include: 1. Nova mandarin + Succari sweet orange 2. Valencia sweet orange + Murcott tangor 3. Succari sweet orange + Murcott tangor 4. Rohde Red Valencia + Dancy mandarin 5. Succari + Page tangelo 6. Valencia + Page tangelo 7. Succari + Minneola tangelo 8. Hamlin sweet orange + Ponkan mandarin 9. Hamlin + LB8-4 (Clementine x Minneola) 10. Valencia + (Robinson x Temple) 11. Pink Marsh grapefruit + Murcott 12. Succari + Dancy 13. Murcott + LB8-9 tangelo 14. Nova + Osceola mandarin Interploid hybridization using tetraploid somatic hybrids as pollen parents to produce seedless triploids for mandarin improvement:- more than 12000 triploids produced to date,many fathered by somatic hybrids (under direction of FG Gmitter,CREC)- oldest hybrids are now fruiting and most are seedless!!!!! NOVA + SUCCARI SOMATIC HYBRID FRUIT (father of several hundred triploid progeny) Valencia + (Robinson x Temple) harvested January 22,2004 brix=11.4, acid=0.57, ratio=20 3 seeds/fruit Rohde Red Valencia + Dancy harvested January 28,2004 brix=11.4, acid=1.57, ratio= 7.26 3 seeds/fruit Nova + Osceola harvested December 6, 2005 brix= 14.8, acid=1.15, ratio= 12.9 EMBRYO RESCUE- Facilitates triploid plant recovery from interploid crosses (circumvents embryo abortion due to endosperm failure)- Also useful for triploid recovery exploiting 2n gametes (unreduced) from cultivars such as Clementine and Temple (several thousand triploids recovered at IVIA, Spain) New triploid mandarin hybrids following embryo rescue and micro-grafting (F.G. Gmitter, Jr.)-...
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GAM 2009 - Applications of Somatic Hybridization and...

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