Genetic Transformation 1 2009

Genetic Transformation 1 2009 - Plant Genetic...

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Plant Genetic Transformation
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All stable transformation methods consist of three steps: Delivery of DNA into a single plant cell. Integration of the DNA into the plant cell genome. Conversion of the transformed cell into a whole plant.
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Methods of delivering DNA into plant cells Biological Agrobacterium Other bacteria Viruses Physical Particle bombardment Electroporation Silicon carbide whiskers Carbon nanofibers
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Agrobacterium -mediated Transformation
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Biology of the Agrobacterium -plant interaction The only known natural example of inter-kingdom DNA transfer
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Infects at root crown or just below the soil line.  Can survive independent of plant host in the soil.  Infects plants through breaks or wounds. Common disease of woody shrubs, herbaceous plants,  dicots.  Galls are spherical wart-like structures similar to  tumors.
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The genus Agrobacterium has a wide host range: Overall, Agrobacterium
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Genetic Transformation 1 2009 - Plant Genetic...

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