Unformatted text preview: bleach solution. 5. Rinse the carrots three times with sterile water (100 – 150ml), covering the sections and stirring for 1-2 min each time. 6. Transfer a section into a sterile dish. Using scalpel and forceps, cut small (5mm square) pieces of root, making sure to include the cambium (the innermost ‘ring’ of the root). Sterilize Instruments often. 7. Place 4 explants in each of the provided Petri plates. You will be given two types of plates - containing MS medium and MS medium + 2,4-D (prepared on 1/5). Prepare one of each media type for each root treatment. 8. Place 4 of the slices into a Petri plate with sterile filter paper which has been moistened with sterile distilled water. 9. Seal each dish with parafilm, label and incubate in the dark. 10. After 4 weeks, subculture the callus onto the same medium, discarding necrotic tissue. 11. After another 4 weeks, transfer all tissue to hormone-free medium (MS media without 2,4-D)....
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- Spring '09
- carrot, distilled water, Petri dish