Lab 03-cauliflower_coleus

Lab 03-cauliflower_coleus - Lab 3b Producing Plantlets from...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Lab 3a Production of Plantlets from Flower Organs of Cauliflower Protocol: 1. Outside of the hood, select a clean floret from a fresh cauliflower head. Using forceps and scalpel, trim off 12-15 small pieces of florets for explants 2. In the flow hood, transfer the material into a sterile beaker containing a stir bar 3. Add 70% EtOH and stir for 5 min. Pour off EtOH 4. Add 20% bleach + Tween 20 and stir for 5 min. Pour off Bleach 5. Rinse tissue 3X with Sterile water (1-2min each) 6. Transfer the explants into 2 petri plates containing MS medium AND 2 petri plates containing MS medium supplemented with 2.5 mg/L of kinetin. 7. Seal the plates, label them and maintain them in the light. 8. Observe plants weekly 9. When shoots develop, in 6-8 weeks, separate from mass at base of the shoots and put them into hormone free medium. Roots should form in 2-3 weeks.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Lab 3b Producing Plantlets from Coleus leaves 1. Leaves from Coleus plants have been collected and provided to you. It is your objective to regenerate shoots that are of a single color how would you go about doing that with these Coleus leaves? 2. In the flow hood, transfer the material into a sterile beaker containing a stir bar 3. Add 70% EtOH and stir for 3 min. Pour off EtOH 4. Add 20% bleach + Tween 20 and stir for 5 minutes Maximum. Pour off Bleach. 5. Rinse tissue 3X with Sterile water (1-2min each) 6. Lay a leaf out on the inside of a large petri plate. Cut the leaf into 8-10 pieces. 7. Transfer the explants into 2 petri plates containing MS medium plus 2 mg/l BA & 1 mg/l NAA. 8. Seal the plates, label them and maintain them in the light. 9. Observe plants weekly....
View Full Document

This note was uploaded on 07/23/2011 for the course HOS 6737c taught by Professor Moore during the Spring '09 term at University of Florida.

Page1 / 2

Lab 03-cauliflower_coleus - Lab 3b Producing Plantlets from...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online