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Unformatted text preview: 6. Resuspend the seeds in 0.1% (w/v) sterile phytoblend agar. 7. Distribute the seeds on plates using the pipet. 8. Wrap the seeds with parafilm and store at 4 C for 48 hours. 9. Transfer to growth chamber (20 C) 10. After a few weeks, observe the ratio of viable to non-viable seedlings Arabidopsis Growth Media (per Liter) 4.4g MS salts & vitamins, buffered 10g Sucrose pH 5.7 8g Phytoblend agar 50mg Kanamycin 30...
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- Spring '09