Unformatted text preview: – IT IS A CARCINOGEN!!! 7. Remove comb, place tray into gel apparatus and fill with 1X TAE buffer until gel is covered. 8. Pipet 10ul of DNA ladder into the first lane (prepared for you), and follow suit with PCR sample in remaining lanes. 9. Put on apparatus cover and run gel at 100V for 30 minutes. 10. Using the imaging apparatus, take a picture of the gel under UV light. MAKE SURE THE UV LIGHT IS COVERED- do not expose eyes to UV light! 11. Compare your PCR product with the 1Kb ladder to determine the size of the band....
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- Spring '09
- DNA, Erlenmeyer flask, 1X TAE buffer