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Unformatted text preview: IT IS A CARCINOGEN!!! 7. Remove comb, place tray into gel apparatus and fill with 1X TAE buffer until gel is covered. 8. Pipet 10ul of DNA ladder into the first lane (prepared for you), and follow suit with PCR sample in remaining lanes. 9. Put on apparatus cover and run gel at 100V for 30 minutes. 10. Using the imaging apparatus, take a picture of the gel under UV light. MAKE SURE THE UV LIGHT IS COVERED- do not expose eyes to UV light! 11. Compare your PCR product with the 1Kb ladder to determine the size of the band....
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This note was uploaded on 07/23/2011 for the course HOS 6737c taught by Professor Moore during the Spring '09 term at University of Florida.
- Spring '09