Lab Safety and Sterile Technique 2009

Lab Safety and Sterile Technique 2009 - LAB SAFETY Apparel...

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LAB SAFETY
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Apparel Shoes Lab coats Safety glasses
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Note! No eating, drinking, or smoking in the lab at any time.
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Spills Wash Notify Receive instructions
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Accidents Be careful! Pay attention Report any injuries
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Other Matters Broken glass Other waste Pipetting Volatile chemicals Dirty labware Labeling General cleanliness
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ASEPTIC TECHNIQUE
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I. Contaminants A. Bacteria, fungi, and insects B. Initial contaminants C. Latent or persistent contamination D. Introduced contamination E. Detection of contaminants
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THE TRANSFER HOOD The hood should remain on Necessary items present, unnecessary things removed Check the bottom of the hood for blockage Wash hands  
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THE TRANSFER HOOD Spray or wipe the inside of the transfer hood Wipe hands and lower arms with 70% EtOH Remove dangling sleeves and jewelry
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Sterilization and use of supplies and equipment Know which of your implements, flasks, etc. are sterile and which are not
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