Media Preparation from Basal Stock Solutions

Media Preparation from Basal Stock Solutions -...

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Phyto Technology Laboratories , Inc. P.O. Box 13481; Shawnee Mission, KS 66282-3481 Phone: 1-888-749-8682 or 913-341-5343; Fax: 1-888-449-8682 or 913-341-5442 Web Site: www.phytotechlab.com Dedicated to a Better Way of Life through Plants TM Phyto Technology Laboratories, Inc . Product Information Sheet MEDIA PREPARATION FROM BASAL SALT SOLUTIONS Liquid 10X solutions are offered for your convenience. To avoid precipitation over long-term storage, Phyto Technology Laboratories has formulated two solutions which when mixed at the proper dilution make a solution with the appropriate salt concentration. The basic steps for preparing 1 liter of culture medium are listed below. CAUTION: Do not autoclave product in bottle. The bottle is NOT autoclavable. 1. Measure out approximately 700 ml of tissue culture grade water. 2. While stirring the water, add 100 ml of Macronutrient Solution (Product No. M 654) 3. Continue stirring the mixture while adding 100 ml of Micronutrient Solution (Product No. M 529)
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This note was uploaded on 07/23/2011 for the course HOS 6737c taught by Professor Moore during the Spring '09 term at University of Florida.

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Media Preparation from Basal Stock Solutions -...

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