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Media - Plant Tissue Culture W hat is it Tissue culture is...

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Plant Tissue Culture
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What is it? Tissue culture is the term used for “the process of growing cells artificially in the laboratory” Tissue culture produces clones , in which all product cells have the same genotype (unless affected by mutation during culture)
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Why does it work? Plant cells – Dedifferentiate Plant cell division- Somatic cells are diploid Mitosis – Chromosomes duplicate and form clones Totipotency
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What’s the history? Cell theory, suggesting totipotentiality of cells. Schleiden M. J., Arch. Anat., Physiol. U. wiss. Med. (J. Muller), 1838: 137-176; Schwann T., W. Engelman, No. 176 (1910)
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What’s the history? 1902 - First but unsuccessful attempt of tissue culture using monocots. Haberlandt G., Sitzungsber Akad. Wiss. Wien, Math.- Naturwiss. Kl., 111: 69-92. 1926 - FW Went demonstrated that there were growth substances in coleoptiles from Avena
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What’s the history? 1934 - White generated continuously growing culture of meristematic cells of tomato on medium containing salts,yeast extract and sucrose and 3 vitamins (pyridoxine, thiamine, nicotinic acid) – established the importance of additives
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What’s the history? 1939 - Successful continuously growing cambial cultures of carrot and tobacco. Gautheret R. J., C. R. Acad. Sci. (Paris), 208: 118-120; Nobecourt P., C. R. Soc. Biol. (Paris), 130: 1270-1271; White P. R., Am. J. Bot., 26: 59-64
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What’s the history? 1943-1950 - Tumor-inducing principle of crown gall tumors identified. Braun A. C. Phytopathol. 33: 85-100 & P. N. A. S. USA 45: 932-938 1948 - Formation of adventitious shoots and roots in tobacco. Skoog F. and Tsui C., Am. J. Bot., 355: 782-787
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What’s the history?
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