More details on Agrobacterium

More details on Agrobacterium - All stable transformation...

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1 Plant Genetic Transformation All stable transformation methods consist of three steps: ± Delivery of DNA into a single plant cell. ± Integration of the DNA into the plant cell genome. ± Conversion of the transformed cell into a whole plant. Agrobacterium -mediated Transformation Biology of the - plant interaction The only known natural example of inter-kingdom DNA transfer
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2 •Infects at root crown or just below the soil line. •Can survive independent of plant host in the soil. •Infects plants through breaks or wounds. •Common disease of woody shrubs, herbaceous plants, dicots. •Galls are spherical wart-like structures similar to tumors. The genus Agrobacterium has a wide host range: ± Overall, can transfer T-DNA to a broad group of plants. ± Yet, individual strains hve a limited host range. ± The molecular basis for the strain-specific host range is unknown. ± Many monocot plants can be transformed (now), although they do not form crown gall tumors. ± Under lab conditions, T-DNA can be transferred to yeast, other fungi, and even animal and human cells. Why is used for producing transgenic plants? ± The T-DNA element is defined by its borders but not the sequences within. So researchers can substitute the T-DNA coding region with any DNA sequence without any effect on its transfer from Agrobacterium into the plant. Steps of Agrobacterium-plant cell interaction ± Cell-cell recognition ± Signal transduction and transcriptional activation of vir genes ± Conjugal DNA metabolism ± Intercellular transport ± Nuclear import ± T-DNA integration
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T-DNA ± T-DNA carries genes involved in the synthesis of plant growth hormones (auxin, auxin synthesis; cyt, cytokinin synthesis) and the production of low molecular weight amino acid and sugar phosphate derivatives called opines (ocs, octopine; mas, mannopine; and ags, agropine).Agrobacteria are usually classified based on the type of opines specified by the bacterial T-DNA. Ti Plasmid
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More details on Agrobacterium - All stable transformation...

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