More information on biolistics

More information on biolistics - CSS/HRT 451 CSS/HRT 451...

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: CSS/HRT 451 CSS/HRT 451 CSS/HRT 451 CSS451 Biolistic-mediated transformation Guo-qing Song Guo Guo-qing qing Song Song f Gene Delivery System y Agrobacterium y Viral vectors y Biolistic y Microinjection y PEG - Polyethylene Glycol y Electroporation f Advantage: y This method can be use to transform all plant species. y No binary vector is required. y Transformation protocol is relatively simple. f Disadvantage: y Difficulty in obtaining single copy transgenic events. y High cost of the equipment and microcarriers. y Intracellular target is random (cytoplasm, nucleus, vacuole, plastid, etc.). y Transfer DNA is not protected. Biolistic Biolistic Transformation Transformation------- Advantage and Disadvantage Known as: Particle Bombardment Biolistics Microprojectile bombardment Particle acceleration Particle inflow gun Gene gun Using a gene gun directly shoots a piece of DNA into the recipient plant tissue. Tungsten or gold beads are coated in the gene of interest and fired through a stopping screen, accelerated by Helium, into the plant tissue. The particles pass through the plant cells, leaving the DNA inside. Gene Delivery System------Biolistic-mediated transformation Biolistic Biolistic-Mediated Gene Transfer Mediated Gene Transfer------- Mechanism The Helios Gene Gun http://www.oardc.ohio-state.edu/plantranslab/PIG.htm www.bio-rad.com/genetransfer/ PDS-1000/He Particle Inflow Guns (PIG) Biolistic Biolistic-Mediated Gene Transfer Mediated Gene Transfer------- Equipment www.bio-rad.com/genetransfer/ PDS-1000/He DNA-coated microcarriers are loaded on microcarrier. Micro-carriers are shot towards target tissues during helium gas decompression. A stopping screen placed allowing the coated microprojectiles to pass through and reach the target cells. Biolistic Biolistic-Mediated Gene Transfer Mediated Gene Transfer------- Equipment- PDS-1000/He Arnold D et al., Proc Natl Acad Sci USA 91, 9970–9974 (1994) Castillo AM et al., Biotechnology 12, 1366–1371 (1994) Duchesne LC et al., Can J For Res 23, 312–316 (1993) Fitzpatrick-McElligott S, Biotechnology 10, 1036–1040 (1992) Hartman CL et al., Biotechnology 12, 919–923 (1994)...
View Full Document

{[ snackBarMessage ]}

Page1 / 19

More information on biolistics - CSS/HRT 451 CSS/HRT 451...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online