Plant Growth Rgulators Used in Plant Tissue Culture

Plant Growth Rgulators Used in Plant Tissue Culture -...

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Plant Growth Regulators Commonly Used in Plant  Tissue Culture Class Name Abbreviation Comments Auxin Indole-3-acetic acid IAA Use for callus induction at 10-30 µM.  Lowering to 1-10 µM can stimulate  organogenesis. Is inactivated by light  and readily oxidized by plant cells.  The synthetic auxins below have  largely superceded IAA for tissue  culture studies.   Indole-3-butyric acid IBA Use for rooting shoots regenerated  via organogenesis. Either maintain at  a low concentration (1-50 µM)  throughout the rooting process, or  expose to a high concentration (100- 250 µM) for 2-10 days and then  transfer to hormone-free medium.  Can also use as a dip for  in vitro  or  ex vitro  rooting of shoots.    2,4-Dichlorophenoxyacetic  acid 2,4-D Most commonly used synthetic auxin  for inducing callus and maintaining  callus and suspension cells in  dedifferentiated states. Usually used 
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Plant Growth Rgulators Used in Plant Tissue Culture -...

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