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PREPARATION OF SEED GROWING MEDIA - 6 Add 10 grams of agar...

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PREPARATION OF SEED GROWING MEDIA SHOOT TIP GRAFTING SOLID MEDIA TUBES 1. In a clean beaker add 900ml. of deionized water and 1 package of Murashige and Skoog salts 2. Adjust pH to 5.6 – 5.8 using 1N KOH 3. Place in microwave and bring to a boil 4. Bring solution to 1 liter with deionized water 5. On the hot plate bring solution to boil
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Unformatted text preview: 6. Add 10 grams of agar while stirring 7. When agar has dissolved (solution should be clear) dispense ~ 25ml per tube 8. Cap tubes !! if cap is not on tube firm (tube can be picked up by cap) change tube!! 9. Wrap basket in aluminum foil and place autoclave tape on foil 10. Autoclave at 121ºC for 15 minutes...
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