Sterilizing Nutrient Media

Sterilizing Nutrient Media - PhytoTechnology Laboratories...

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Phyto Technology Laboratories , Inc. P.O. Box 13481; Shawnee Mission, KS 66282-3481 Phone: 1-888-749-8682 or 913-341-5343; Fax: 1-888-449-8682 or 913-341-5442 Web Site: Dedicated to a Better Way of Life through Plants TM Phyto Technology Laboratories, Inc . Product Information Sheet Sterilizing Nutrient Media Two methods (autoclaving and membrane filtration under positive pressure) are commonly used to sterilize culture media. Culture media, distilled water, and other stable mixtures can be autoclaved in glass containers that are sealed with cotton plugs, aluminum foil, or plastic closures. However, solutions that contain heat-labile components must be filter-sterilized. Generally, nutrient or plant tissue culture media are autoclaved at 1.05 kg/cm 2 (15 psi) and 121 ° C. The time required for sterilization depends upon the volume of medium in the vessel. For small volumes of liquids (100 ml or less), the time required for autoclaving is 15-20 min, but for larger quantities (2-4 liter), 30-40 min is required.
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