Paper35-Histological-biological-effects-granulation-tissue-loosened hip-development-osteolysis-J-Ort

2 immunohistochemical staining for cd68 interleukin 6

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Unformatted text preview: dy ( 200); c multinuclear giant cells (hematoxylin and eosin, 200); d polarized light photomicrograph (hematoxylin and eosin, 200) Fig. 2. Immunohistochemical staining for CD68, interleukin-6 (IL-6) and tumor necrosis factor (TNF)-α. The negative control was immunostained for nonimmune mouse immunoglobulin ( 100) 482 S. Ito et al.: Granulation tissue in osteolysis a b c d particles, we examined their distribution semiquantitatively in all samples of capsules and periprosthetic granulation tissues. The histological findings were similar in tissue specimens from around the stem and the socket, as well as in a capsule. Histiocytes were more abundant than giant cells. No particles were identified in about 30%–40% of periprosthetic tissues, and in 60% of capsules (Fig. 3). We compared the relationship between giant cells and polyethylene particles and found that the amount of particles correlated with giant cells (r 0.797, P 0.0001), but not with histiocytes (Fig. 4). Among the clinical findings such as age and duration of implantation, the type of fixation was strongly related to the histol...
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