Paper35-Histological-biological-effects-granulation-tissue-loosened hip-development-osteolysis-J-Ort

20 s ito et al granulation tissue in osteolysis 485

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Unformatted text preview: s. However, it has been recently reported that contact between wear particles and macrophages without phagocytosis is also important for the signal transduction of cytokines.20 S. Ito et al.: Granulation tissue in osteolysis 485 Fig. 7. Reverse transcriptase-polymerase chain reaction (RTPCR) analysis of interleukin (IL)-6, tumor necrosis factor (TNF)-α, osteoprotegerin (OPG), osteoclast differentiation factor (ODF), and matrix metalloproteinase (MMP)-13 in the granulation tissue of massive osteolysis. Molecular weight markers (bp) are shown on the left Fig. 8. Effect of conditioned medium from granulation organ culture on interleukin (IL)-6 production by osteoblasts. Ten or 20 µl of conditioned medium of the granulation tissue was added to 1 ml of osteoblast culture medium. IL-6 was not detected in the medium before the culture. After 24 h of culture, the concentration of IL-6 was measured. Values are the mean SEM Fig. 9. Effects of the antibodies for interuleukin (IL)-1 or tumor necrosis factor (TNF)-α, indomethacin, and dexamethasone on the production of IL-6 from osteoblasts stimulated by conditioned medium from granulation tissue culture. Ten microliters of conditioned medium was added to 1 ml...
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This note was uploaded on 07/25/2011 for the course EMA 6580 taught by Professor Staff during the Spring '08 term at University of Florida.

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