This preview shows page 1. Sign up to view the full content.
Unformatted text preview: s. However,
it has been recently reported that contact between wear
particles and macrophages without phagocytosis is also
important for the signal transduction of cytokines.20 S. Ito et al.: Granulation tissue in osteolysis 485 Fig. 7. Reverse transcriptase-polymerase chain reaction (RTPCR) analysis of interleukin (IL)-6, tumor necrosis factor
(TNF)-α, osteoprotegerin (OPG), osteoclast differentiation factor (ODF), and matrix metalloproteinase (MMP)-13 in the
granulation tissue of massive osteolysis. Molecular weight
markers (bp) are shown on the left Fig. 8. Effect of conditioned medium from granulation organ
culture on interleukin (IL)-6 production by osteoblasts. Ten
or 20 µl of conditioned medium of the granulation tissue was
added to 1 ml of osteoblast culture medium. IL-6 was not
detected in the medium before the culture. After 24 h of culture, the concentration of IL-6 was measured. Values are the
mean SEM Fig. 9. Effects of the antibodies for interuleukin (IL)-1 or
tumor necrosis factor (TNF)-α, indomethacin, and dexamethasone on the production of IL-6 from osteoblasts stimulated by conditioned medium from granulation tissue culture.
Ten microliters of conditioned medium was added to 1 ml...
View Full Document
- Spring '08