Paper35-Histological-biological-effects-granulation-tissue-loosened hip-development-osteolysis-J-Ort

486 fig 10 reverse transcriptase polymerase chain

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Unformatted text preview: tissue seem to interplay in vivo. We therefore examined the effect of granulation tissue on the osteoblasts and found that IL-6 was produced not only by macrophages but also by osteoblasts. 486 Fig. 10. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of interleukin (IL)-6 and collagen I (COL I) in osteoblasts. Total RNA was extracted 24 h after treatment with ( ) or without ( ) conditioned medium from granulation tissue culture. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was an internal control In summary, macrophages in granulation tissues were stimulated by particles or mechanical stress to produce several kinds of cytokines, MMPs, and other chemical mediators. These may affect the activity of both osteoblasts and osteoclasts, leading to osteolysis. Acknowledgment. The authors are grateful to Dr. H Oonishi. (Memorial Joint Replacement Institute, Tominaga Hospital, Osaka, Japan) for providing samples of granulation tissue from patients with aseptic loosening. Referen...
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