Unformatted text preview: tissue seem to interplay in
vivo. We therefore examined the effect of granulation
tissue on the osteoblasts and found that IL-6 was produced not only by macrophages but also by osteoblasts. 486 Fig. 10. Reverse transcriptase-polymerase chain reaction
(RT-PCR) analysis of interleukin (IL)-6 and collagen I (COL
I) in osteoblasts. Total RNA was extracted 24 h after treatment with ( ) or without ( ) conditioned medium from
granulation tissue culture. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was an internal control In summary, macrophages in granulation tissues were
stimulated by particles or mechanical stress to produce
several kinds of cytokines, MMPs, and other chemical
mediators. These may affect the activity of both osteoblasts and osteoclasts, leading to osteolysis.
Acknowledgment. The authors are grateful to Dr. H Oonishi.
(Memorial Joint Replacement Institute, Tominaga Hospital,
Osaka, Japan) for providing samples of granulation tissue
from patients with aseptic loosening. Referen...
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- Spring '08
- Hip replacement, ET AL, osteolysis, granulation tissue, polyethylene particles