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Unformatted text preview: s (sample number 82);
b capsule (sample number
23); c granulation tissue
number 36); and d granulation tissue around stems
(sample number 23). Histologic grading was as follows:
0 absent; 1 rare, 10%;
occasional, 10%–30%; 3
moderate, 30%–50%; and
4 frequent, 50% of ﬁelds Fig. 4. Correlation between the histological grade of
polyethylene particles and giant cells in the retrieved granulation tissue. Spearman’s rank correlation was 0.797 strated in the retrieved periprosthetic granulation tissue
(Fig. 6). The granulation tissue expressed IL-6, TNF-α,
OPG, ODF, and MMP-13 mRNA in RT-PCR (Fig. 7).
To determine the biological effects of granulation tissue
in osteolysis, 1 g of granulation tissue was organ cultured in 10 ml of DMEM for 2 days. The concentrations
of IL-6 and TNF-α were 2700 pg/ml and 4 pg/ml, respectively. Ten or 20 µl of this medium was added to 1 ml of
osteoblast culture medium, and was thus diluted 100 or S. Ito et al.: Granulation tissue in osteolysis 483 Table 3. Histological data of retrieved tissues from 40 loosened hip prostheses assessed se...
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- Spring '08