Paper35-Histological-biological-effects-granulation-tissue-loosened hip-development-osteolysis-J-Ort

Il 6 was not detected in the medium before the

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Unformatted text preview: of osteoblast culture medium, alone or in combination with 10 µg/ml antibody for IL-1 (IL-1 Ab) or for TNF-α (TNF Ab), 10 4 M indomethacin (Ind), or 10 6 M dexamethasone (DEX). IL-6 was not detected in the medium before the culture. After 24 h of culture, the IL-6 levels were measured. Values are the mean SEM. Asterisks denote a significant difference compared with treatment by conditioned medium alone (* P 0.05, ** P 0.01) We compared the histological grading with the clinical findings and found more particles in tissue around a noncemented socket. Although the histological differences between cemented and noncemented implants are controversial,7 Jones et al.12 showed that immunohistochemical staining for cytokines is similar in both groups. Several cytokines, such as IL-6, TNF-α, and IL1 , have been identified immunohistologically or by in situ hybridization.7,11,12,25 These cytokines are considered to have been produced by particle-stimulated macrophages and to have played a role in bone resorption. In agreement with this, w...
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This note was uploaded on 07/25/2011 for the course EMA 6580 taught by Professor Staff during the Spring '08 term at University of Florida.

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