Paper35-Histological-biological-effects-granulation-tissue-loosened hip-development-osteolysis-J-Ort

Monoclonal anti cd68 anti body was obtained from

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Unformatted text preview: ectastain kit (Vector Laboratories, Burlingame, CA, USA). Monoclonal anti-CD68 anti- body was obtained from ZYMED (ZYMED Laboratories, San Francisco, CA, USA). Monoclonal anti-human TNF-α and monoclonal anti-human IL-6 antibodies were purchased from DAKO (Kyoto, Japan). Nonimmune mouse immunoglobulin was used as a negative control for nonspecific binding reactivity. Polyethylene particles were examined under polarized light. Histological grading was evaluated using a quantitative five-grade (0–4) system for the presence of giant cells, histiocytes, and polyethylene particles as described by Goodman et al.:6 0 absent; 1 rare, 10% of fields; 2 occasional, 10–30% of fields; 3 moderate, 30%–50% of fields; and 4 frequent, 50% of fields. 480 S. Ito et al.: Granulation tissue in osteolysis Table 2. Primer sequences for reverse transcriptase-polymerase chain reaction Gene GAPDH IL-6 TFN-α ODF OPG Collagen I MMP-13 Sequences 5 5 5 5 5 5 5 5 5 5 5 5 5 5 Size -GCTCTCCAGAACATCATCCCTGCC-3 -CGTTGTCATACCAGGAAATGAGCTT-3 -ATGAACTCCTTCTCCACAAG-3 -GTGCCTGCAGCTTCGTCAGCA-3 -TCAGATCATCTTCTCGAACC-3 -CAGATAGAGATGGGCTCATACC-3 -ATAGAATATCAGAAGATGGCACTC-3 -TAAGGAGGGGTTGGAGACCTCG-3 -TGCTGTTCCTACAAAGTTTACG-3 -CTTTGAGTGCTTTAGTGCCTG-3 -AGCGCTGGTTTCGACTTCAGCTTCC-3 -CATCGGCAGGGTCGGAGCCCT-3 -GACTTCACGATGGCATTGCTG-3 -GCATCAACCTGCTGAGGATGC-3 346 546 361 668 435 340 491 GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL-6, interleukin-6; TNF, tumor necrosis factor; ODF, osteoclast dif...
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