Paper35-Histological-biological-effects-granulation-tissue-loosened hip-development-osteolysis-J-Ort

The primer sequences are shown in table 2 statistical

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Unformatted text preview: °C for 1 min. All final elongation steps were performed at 72°C for 1 min. PCR products were electrophoresed on 2% agarose gel. The primer sequences are shown in Table 2. Statistical analysis The Mann-Whitney U test was used to compare the differences between samples. Spearman’s rank correlation was used to analyze the relationship among different parameters. Results The histological findings of tissues showed numerous multinucleated giant cells and histiocytes, which were immunohistochemically detected by a CD68 antibody. Various sizes of polyethylene particles were identified in the polarized light (Fig. 1). In addition, these histiocytes were immunostained with an IL-6 or TNF-α antibody (Fig. 2). To clarify the heterogeneity in the distribution of histiocytes, giant cells, and polyethylene S. Ito et al.: Granulation tissue in osteolysis a c 481 b d Fig. 1a–d. Photomicrograph of granulation tissue around a loosened prosthesis. a Histiocyte (hematoxylin and eosin, 200); b immunostaining for macrophages using anti-CD68 antibo...
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This note was uploaded on 07/25/2011 for the course EMA 6580 taught by Professor Staff during the Spring '08 term at University of Florida.

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