Unformatted text preview: °C
for 1 min. All ﬁnal elongation steps were performed at
72°C for 1 min. PCR products were electrophoresed on
2% agarose gel. The primer sequences are shown in
The Mann-Whitney U test was used to compare the
differences between samples. Spearman’s rank correlation was used to analyze the relationship among different parameters. Results
The histological ﬁndings of tissues showed numerous
multinucleated giant cells and histiocytes, which were
immunohistochemically detected by a CD68 antibody.
Various sizes of polyethylene particles were identiﬁed in the polarized light (Fig. 1). In addition, these
histiocytes were immunostained with an IL-6 or TNF-α
antibody (Fig. 2). To clarify the heterogeneity in the
distribution of histiocytes, giant cells, and polyethylene S. Ito et al.: Granulation tissue in osteolysis a c 481 b d Fig. 1a–d. Photomicrograph of granulation tissue around a loosened prosthesis. a Histiocyte (hematoxylin and
eosin, 200); b immunostaining for
macrophages using anti-CD68 antibo...
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This note was uploaded on 07/25/2011 for the course EMA 6580 taught by Professor Staff during the Spring '08 term at University of Florida.
- Spring '08