Paper51-Osteoblast-spreading-silica-patterns-Microscopy-Pelaez-Vargas-2010

Paper51-Osteoblast-spreading-silica-patterns-Microscopy-Pelaez-Vargas-2010

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Early Spreading and Propagation of Human Bone Marrow Stem Cells on Isotropic and Anisotropic Topographies of Silica Thin Films Produced via Microstamping A. Pelaez-Vargas, 1,2, * D. Gallego-Perez, 2 N. Ferrell, 2 M.H. Fernandes, 3 D. Hansford, 2 and F.J. Monteiro 1 1 INEB - Instituto de Engenharia Biomédica and Universidade do Porto, Faculdade de Engenharia, Departamento de Engenharia Metalúrgica e Materiais, Porto, Portugal 2 Department of Biomedical Engineering, The Ohio State University, Columbus, OH, USA 3 Universidade do Porto, Faculdade de Medicina Dentária, Laboratório de Farmacologia e Biocompatibilidade, Porto, Portugal Abstract: While there has been rapid development of microfabrication techniques to produce high-resolution surface modifications on a variety of materials in the last decade, there is still a strong need to produce novel alternatives to induce guided tissue regeneration on dental implants. High-resolution microscopy provides qualitative and quantitative techniques to study cellular guidance in the first stages of cell-material interactions. The purposes of this work were ~ 1 ! to produce and characterize the surface topography of isotropic and anisotropic microfabricated silica thin films obtained by sol-gel processing, and ~ 2 ! to compare the in vitro biological behavior of human bone marrow stem cells on these surfaces at early stages of adhesion and propagation. The results confirmed that a microstamping technique can be used to produce isotropic and anisotropic micropatterned silica coatings. Atomic force microscopy analysis was an adequate methodology to study in the same specimen the sintering derived contraction of the microfabricated coatings, using images obtained before and after thermal cycle. Hard micropatterned coatings induced a modulation in the early and late adhesion stages of cell-material and cell-cell interactions in a geometry-dependent manner ~ i.e., isotropic versus anisotropic ! , as it was clearly determined, using scanning electron and fluorescence microscopies. Key words: silica sol-gel films, soft lithography, microstamping, cell adhesion and proliferation, SEM, AFM I NTRODUCTION Dental implants as an alternative treatment for lost teeth are not free of controversy ~ Sennerby, 2008 ! , and important differences may be found when comparing experimental and clinical results ~ Albrektsson et al., 2008 ! . In general, it is well accepted that macroroughness provides primary stabilization for dental implants in the post-surgical phase, and that slight microroughness has some clinical advantages ~ Wennerberg et al., 1995; Albrekts- ! . However, results of these studies are often not statistically significant. Recently, the interest in nanoscale roughness is growing ~ Meirelles et al., 2008 ! ,as protein adsorption occurs at this scale, which is a key step in the process of osseointegration. To date, no randomized controlled clinical trial has been reported supporting supe-
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This note was uploaded on 07/25/2011 for the course EMA 6580 taught by Professor Staff during the Spring '08 term at University of Florida.

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Paper51-Osteoblast-spreading-silica-patterns-Microscopy-Pelaez-Vargas-2010

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