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lecture 25 - Lecture 25 Monday Announcements We have Lab#4...

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Lecture 25. Monday 3-28-11
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Announcements We have Lab #4 this week ( 3-28 to 4-1) . Exam #2 will be on Wednesday, March 30 Last day to drop (R) is Friday 4-1-11 The Final Exam is on Tuesday May 10 from 11:45 to 2:45 in Knox 20 My Help Session for Exam II will be from 5:00 to 6:50PM on Tuesday (3-29-11) in C121 Cooke Hall
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Exam II 1. The next exam is on March 30. 2. It will cover information from Lecture 11 (on 2-14) through Lecture 24 (on 3-25). 3. No lab material on this exam
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Undergraduate TA Help Sessions Monday 10:00AM C651 Cooke Hall Monday 5:00 C651Cooke Hall Thursday 5:00 C651Cooke Hall Friday 2:00 C651Cooke Hall His office hours are 11:00 to 12:00 on Tuesdays in H631 Hochstetter Hall [email protected] The Graduate TA is Dan Samorodinitsky My Help Session for Exam II will be from 5:00 to 6:50 on Tuesday (3-29-11) in C121 Cooke Hall
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Lab # 4 Lab #4 is this week (3-2811 to 4-1-11) for BIO201B
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Objectives for Lab #4: 1. Use homogenization and differential centrifugation to obtain live, functioning chloroplasts. 2. Use DCIP as an artificial electron acceptor to report on the light-driven electron transport activity of these chloroplasts. 3. Determine whether or not the inhibitor DCMU can stop electron transport in chloroplasts
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Centrifugation dx dt = Rate of sedimentation = 2r 2 P – ρ m ) x g = velocity r = radius of the particle (size) ρ P = density of the particle. Lead is more dense than plastic ρ m = density of the medium. High sucrose is more dense than low sucrose g = force of gravity used η = viscosity of the medium. Not the same as density Larger particles have a greater sedimentation rate because they have bigger r.
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