lecture 33 - Lecture 33 Some ways to regulate proteins...

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Lecture 33
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Some ways to regulate proteins involved in the cell cycle 1. Change their amounts A. Change rate of transcription or translation B. Change rate of degredation 2. Change their locations A. Move them, target them, anchor them 3. Change their activites A. Covalent modifications (like phosphorylation)
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An example of changing amounts of a regulatory protein
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Two cyclins in yeast, a model eukaryotic cell G1 Cyclin Mitotic Cyclin G1 cyclin stimulates progression from G1 to S-phase Mitotic cyclin stimulates progression from G2 to mitosis One MPF (cdc kinase) but two different cyclins that can bind to it
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An example of changing the location of a regulatory protein
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The mitotic cyclin B1 accumulates in the nucleus in the start of mitosis 1. Cyclin B1 was labeled with a green fluorescent protein and injected into a HeLa cell that was in G2 phase. It is seen in the cytoplasm but not as much in the nucleus 2. When the cell begins mitosis, the cyclin B1 accumulates in the nucleus
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An example of changing the activity of an existing protein
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Factors allowing and stimulating anaphase SCF (S-phase control factor) helps start S-phase but goes away in anaphase ( ) APC (Anaphase promoting factor) has two types, one peaks during anaphase ( ) and the other starts at anaphase and ends at S-phase ( )
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Telophase How do you know you’re in telophase? Chromatin becomes disperse and clustered near the poles. No mitotic chromosomes seen How many individual pieces of condensed chromatin are there in telophase?
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Is mitosis the end of M-phase? Right after mitosis is finished, what phase of the cell cycle should the cell be in? A. G1
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lecture 33 - Lecture 33 Some ways to regulate proteins...

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