Thin Layer Chromatography Questions

Thin Layer Chromatography Questions - places 5 Unknown code...

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Agustin Garcia Thin Layer Chromatography Questions 2. Silica gel is synthetically made from sodium silicate. Its molecular formula is Na2SiO3. Silica gel is the stationary phase and is more polar than the mobile phase of Ethyl Acetate. Thus, compounds with higher polarities, closer to that of the silica gel, will move slower across the TLC place and will have a lower Rf value. 4. Hexane is less polar than ethyl acetate. Thus, changing the mobile phase from ethyl acetate to a mixture of 50 % ethyl acetate and 50 % hexane would make the mobile phase less polar. Compounds would move less and Rf values would be smaller because the mobile phase is less likely to displace solutes from the silica binding
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Unformatted text preview: places. 5. Unknown code TFH. We concluded that our unknown was composed of all three compounds; caffeine, aspirin and acetaminophen. This was based on our observation of our TLC plate. Our TLC plate yielded three blotches, with Rf values of 0.15, 0.55 and 0.87. The Rf values were correspondent to the Rf values of caffeine, aspirin and acetaminophen. 6. The plate on the left could have more than one spot joined together. Otherwise, the difference in the left plate could be due to the sample being overloaded. Possibly the compound on the left was used in excess and should be diluted in a repeat experiment....
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This note was uploaded on 07/27/2011 for the course CHEM 3361 taught by Professor Staff during the Spring '08 term at Kennesaw.

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