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4Lectures_2006 - Biochemistry 521 Dr Zucai Suo 740 Bio Sci...

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1 Biochemistry 521 Dr. Zucai Suo 740 Bio. Sci. [email protected] 688-3706 (o) Teaching Assistants: Mr. Wade Duym 726 Biological Sciences [email protected] Ms. Soojin Lee 752 Biological Sciences [email protected] Lab Supervisor: Ms. Cynthia Hatfield 868B Bio. Sci. 292-9482 [email protected] 2 Biochemistry 521 Prerequisites: Biochemistry 511 or 613 or equiv and past the placement test Textbooks : Laboratory Manual Biochemical Techniques by Robyt and White Read the required text before coming to the lab! Basis for Grading Two Midterm exams 2x10% Final exam 25% Unannounced Quizzes 15% Unknowns (~ 4) 10% Practical exam 10% Lab reports, techniques, etc. 20% No makeup quizzes 3 Laboratory Reports: 1. Work independently in the preparation of lab reports 2. Computer-generated lab reports 3. All lab reports are due one week after completion of the experiment. 4. Late reports lead to points deduction (1.5 points/day) 5. No report will be accepted if it is more than one week late. 6. Grading for each lab report (10 point total): Introduction (1 point) Experimental methods (1 point) Raw data (1 point) Calculations (1 point) Results (3 points) Discussion (3 points)
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4 Lab Rules 1. Take turns to use equipment 2. Be considerate and be a good citizen 3. Be Cooperative with your partner 4. Dispose trash in proper trash containers (do not throw away used beakers and flasks, leave them in a specific sink). 5. Be gentle to the equipment 6. Follow safety rules 7. Take detail notes 8. Return your pipet set to GTA at the end of each day 5 Session 1 Pipetting and Weighing 6 Plunger Button Tip Ejector Stainless Steel Ejector Arm (removable) Plastic Shaft Disposable Tip Volume Indicator Volume (Stroke) Adjustment Knob P-20 and P-200 share the same tips. P-1000 uses larger tip. Body (high Impact, low temperature coefficient plastic)
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7 Each set: P-20, 0.1-20 µ l P-200, 20-200 µ l P-1000, 200-1000 µ l Quantitative Liquid Transfer Your Cart: 0 Items / $0.00 Graduated cylinders 8 1. Set the desired volume by dialing downward. 2. Attach a new pipet tip firmly 3. Depress the plunger to the first positive stop 4. Holding the pipet vertically, immerse the tip into the sample liquid to a depth of: 1-2 mm for P-20 and P-200 or 2-4 mm for P-1000. 5. Allow the plunger to return slowly to the Up position. Never snap up plunger . 6. Wait for 1-2 seconds to draw a full-volume of sample.
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