Outline of Topics for Midterm I

Outline of Topics for Midterm I - Chapter 1-3 -...

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Chapter 1-3 - Henderson-Hasselbach and Acid-Base problems - General properties of water - ALL the amino acids and how to draw them with a general idea of pKa values for the R groups and the amine and carboxyl groups - How to purify proteins (section 3.6) o Know why we use NH4So4 Chapter 4 - know the primary, secondary, tertiary, and quaternary structures and all their facets (alpha helix, beta sheets) - The Anfinsen Experiment and its significance (section 4.10 is good to read) - The details of Myoglobin and Hemoglobin (READ pp. 114-121). Also, be aware of the nature of its allosteric regulation (this is not discussed specifically but the principles are on p. 152-154) - What do Ramachandran Plots tell you? - Protein Folding (Chaperones) Chapter 5 - know and be aware of the six classes of enzymes (5.1) - Lineweaver Burke plot and how to obtain information from it and what changes its shape, etc. - Competitive Inhibition - Irreversible Inhibition o Diisopropyl fluorophosphates reaction (Fig. 5.15) -
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Outline of Topics for Midterm I - Chapter 1-3 -...

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